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Nisisako Lab
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The lab makes small droplets and particles in narrow channels and separates cells.
AboutAbout the lab
Frontiers of micro- and nanofluidics and interfacial science
The Nisisako Lab studies fabrication and devices based on fluid phenomena at the micro- and nanoscale.
The lab develops techniques to produce uniformly sized small droplets and particles inside microchannels, as well as techniques to sort particles and cells by size.
Going forward, the lab hopes to further expand research in collaboration with medicine and life sciences.
Key terms
- Microchannel
- An extremely narrow channel for liquid carved into a glass or resin chip, in which liquids can be handled with fine control
- Emulsion
- A mixture in which one of two immiscible liquids, such as water and oil, is dispersed as small droplets in the other
- DLD (deterministic lateral displacement)
- A technique that flows liquid through regularly arranged fine pillars to sort particles and cells by size
NewsLab news
Developed a microfluidic chip that 'spatially programs' separation conditions using temperature
Paper accepted in Soft Matter
Assistant Professor Kanno received the Excellent Research Award from the Society for Chemistry and Micro-Nano Systems
Assistant Professor Kanno's paper received the Top Cited Article 2025 award
Assistant Professor Kanno received the Outstanding Reviewer Award from Analytical Sciences
ResearchResearch themes
The lab studies the behavior of fluids in tiny spaces and interfacial phenomena, and uses them to create new bioanalytical methods and new materials. Centered on techniques for manipulating minute droplets and microparticles, it also conducts research in collaboration with medicine and life sciences.
*Illustrative imageObserving phase inversion and nanoemulsions
Water and oil containing water-soluble and oil-soluble surfactants were flowed through a long microchannel, and phase inversion, in which the continuous phase of the liquid switches from oil to water, was observed. The lab also confirmed that nano-sized emulsions form.
*Illustrative imageMulti-stage DLD with temperature-tunable separation conditions
Pillars that swell and shrink with temperature were arranged so that the size cutoff for separation changes at each location along the channel. Cancer cells in diluted blood can be separated from white blood cells and red blood cells and sent to different outlets.
*Illustrative imageGel particles made with micropillars
On a chip with finely arranged pillars in a regular array, the whole process from droplet formation to gelation and transfer from oil to water is carried out on a single chip. The lab produced uniformly sized gel particles and was also able to encapsulate cells.
*Illustrative imageDroplet devices made with a 3D printer
Using an inexpensive desktop 3D printer, the lab built a device that integrates a droplet-forming channel with a pillar array for sorting particles. It produces large droplets and can separate the main droplets from small satellite droplets.
MembersFaculty
The Nisisako Lab consists of faculty members, visiting researchers, and doctoral, master's, and undergraduate students.As of 2026
- ProfessorTakasi NisisakoPublications ↗
- Assistant ProfessorYusuke KannoPublications ↗
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Report an errorAbout the images
The top and research theme images are illustrative and were not taken at the lab. They are cropped from Wikimedia Commons images. Cropped CC BY-SA images are provided under the same CC BY-SA license as the original (version shown on each line).
- Top image: “T Junction Microfluidic Device” Wongbria / CC BY-SA 4.0 (cropped)
- Observing phase inversion and nanoemulsions: “Particles in polydimethylsiloxane (PDMS) oil-in water emulsion seen through an optical microscope” Kushina Dahlia / CC BY 4.0 (cropped)
- Multi-stage DLD with temperature-tunable separation conditions: “Lab on a Chip ( )” National Institute of Standards and Technology / Public domain (cropped)
- Gel particles made with micropillars: “Sodium alginate beads in a beaker” Angel Mathew / CC BY 4.0 (cropped)
- Droplet devices made with a 3D printer: “SLA produced part” Wizard191 / CC BY-SA 3.0 (cropped)
- Related lab (進士研究室): “Science show magnetic levitation” Roberto Arias from London, United Kingdom / CC BY 2.0 (cropped)
- Related lab (高山研究室): “Worm drive ( )” Bernard Spragg. NZ from Christchurch, New Zealand / CC0 (cropped)
- Related lab (金研究室): “Bug 1c” Sandia National Laboratories / Public domain (cropped)
- Related lab (石田研究室): “Microfluidic chip for point-of-care medical devices” FMNLab / CC BY 4.0 (cropped)
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