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Tsuneda Lab
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The lab reveals the little-known side of bacteria and learns to make full use of their abilities.
AboutAbout the lab
Uncovering bacterial physiology and ecology, and building technology to control them.
The Tsuneda Lab works to uncover how bacteria live and develops technologies to draw out their functions.
It integrates "single-cell engineering," which isolates and examines individual cells, with "multicellular engineering," which examines the relationships among cells within populations and tissues.
The subjects are microbial cells and animal cells, and the lab also studies the interactions between the two.
Its work ranges widely from developing methods to elucidating molecular mechanisms and developing applied technologies, aiming to contribute to medicine, health, food, and the environment.
Key terms
- Hard-to-culture microbe
- A microbe that is difficult to grow in laboratory culture media and therefore cannot be taken out and examined directly
- Nitrifying bacteria
- Bacteria that oxidize ammonia to nitrate and play a role in the nitrogen cycle in water and soil
- Bacteriophage
- A virus that infects bacteria, multiplies inside them, and finally destroys them
NewsLab news
A doctoral student received the Best Oral Presentation Award at the 18th Young Researchers' Colosseum of Bacteriology
A doctoral student received the Outstanding Oral Presentation Award at the 18th Young Researchers' Colosseum of Bacteriology
A doctoral student received the Student Oral Presentation Award at the joint meeting of the Phage Research Society and the Japanese Society for Phage Therapy
A master's student received the Outstanding Presentation Award at the 97th General Meeting of the Japanese Society for Bacteriology
An undergraduate student received the American Society for Microbiology Poster Award at the 2nd Young Phage Researchers' Meeting
ResearchResearch themes
The Tsuneda Lab pursues research in cell function engineering based on systems theory, integrating "single-cell engineering," which isolates single cells to examine their functions, and "multicellular engineering," which examines the relationships among individual cells within cell populations and tissues. Research is divided into three groups, with themes set broadly from method development to elucidation of molecular mechanisms and development of applied technologies.
*Illustrative imageIsolation and Culture of Hard-to-Culture Microbes
It is known that only 0.1% or less of the microbes in the environment have been cultured, and that most are difficult to culture. Focusing on nitrifying bacteria as a model of hard-to-culture microbes, the lab has succeeded in obtaining multiple species from various samples. It seeks to answer the question "why are most environmental microbes hard to culture?" from the standpoints of heterogeneity in growth and interactions between different microbial species.
*Illustrative imageMeasurement Technology for Nucleic Acid-Cleaving Enzymes
Among the nucleic acid-related enzymes that underlie biological phenomena, the lab focuses on RNA interferase, which recognizes single-stranded RNA according to its sequence and cleaves it. By advancing the fluorescent probe technology based on fluorescence quenching, a strength of the lab, it has created its own enzyme activity assay, and it aims to apply RNA-cleavage technology using this enzyme to medicine and the environment.
*Illustrative imageCountering Drug-Resistant Bacteria with Phages
Focusing on the problem of drug-resistant bacteria that antibiotics do not work against, the lab studies the functions and properties of bacteriophages that can kill drug-resistant bacteria. Its work includes searching sewage and wastewater for phages that infect multidrug-resistant bacteria spreading around the world, enhancing the bactericidal activity of phages through genetic recombination, and clarifying how resistance to phages arises.
MembersFaculty
The Tsuneda Lab's members consist of faculty, visiting faculty, invited researchers, and doctoral, master's, and undergraduate students.As of 2026
- ProfessorSatoshi Tsuneda
VoicesStudent voices
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Report an errorAbout the images
The top and research theme images are illustrative and were not taken at the lab. They are cropped from Wikimedia Commons images. Cropped CC BY-SA images are provided under the same CC BY-SA license as the original (version shown on each line).
- Top image: “E-coli (Escherichia coli)” Nurgul D / CC BY-SA 4.0 (cropped)
- Isolation and Culture of Hard-to-Culture Microbes: “Gresham Wastewater Treatment Plant aeration basins 2025” PortlandAppraisalBlog / CC BY-SA 4.0 (cropped)
- Measurement Technology for Nucleic Acid-Cleaving Enzymes: “96 Well Plate (NIH BioArt 7)” Courtesy of NIAID Ryan Kissinger / Public domain (cropped)
- Countering Drug-Resistant Bacteria with Phages: “PhiX174” Fdardel / CC BY-SA 3.0 (cropped)
- Related lab (Takeyama Lab): “Petri dish 2” Drhx / CC BY-SA 4.0 (cropped)
- Related lab (Nishida Lab): “Macs killing cancer cell” Susan Arnold (photographer) / Public domain (cropped)
- Related lab (Asahi Lab): “Ibuprofen-crystals-polarized-light-microscopy” Janeklass / CC BY 4.0 (cropped)
- Related lab (Yura Lab): “Myoglobin” AzaToth / Public domain (cropped)
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