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Shirakawa Lab
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A cell biology lab that uses fluorescence to observe calcium responses in living cells.
AboutAbout the lab
Looking inside living cells to explore how information is transmitted.
The Shirakawa Lab studies how information is transmitted inside living cells.
Mainly using mammalian egg cells, the lab investigates cellular mechanisms while observing the behavior of molecules inside the cells.
It focuses in particular on calcium ions inside cells and is also working on developing new bioimaging methods.
The information on the lab's website dates from around 2016.
Key terms
- Bioimaging
- A method of attaching fluorescent markers to molecules inside living cells or tissues and observing them under a microscope.
- Calcium wave
- The phenomenon in which an increase in intracellular calcium concentration triggered by a stimulus propagates like a wave from one end of the cell to the other.
- Calcium oscillation
- The phenomenon in which the intracellular calcium concentration periodically repeats rising and falling.
ResearchResearch themes
The functions of living cells are thought to be realized by an ingenious and complex system made up of functional biomolecules such as proteins and lipids. While observing the behavior of molecules inside cells, this lab probes them using a variety of methods, aiming to clarify how the cellular system works.
*Illustrative imageMechanisms of Intracellular Calcium Responses
The lab investigates the movements and roles of the molecules involved in intracellular calcium ion responses, aiming to clarify the whole picture of how they are regulated. In hamster fertilized eggs, calcium waves that propagate like a wave and calcium oscillations that periodically rise and fall can be observed.
*Illustrative imageDeveloping New Bioimaging Methods
The lab aims to develop a measurement and analysis system that can observe the movements of many molecules at the same time inside living cells. It is trying to build a system that color-codes molecules with fluorescent substances that have different wavelength characteristics, so that each can be distinguished even when the colors mix.
MembersFaculty
The Shirakawa Lab's members consist of faculty, and doctoral, master's, and undergraduate students.As of 2019
- Associate ProfessorHideki Shirakawa
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Report an errorAbout the images
The top and research theme images are illustrative and were not taken at the lab. They are cropped from Wikimedia Commons images. Cropped CC BY-SA images are provided under the same CC BY-SA license as the original (version shown on each line).
- Top image: “Calcium fluorescence imaging schematic” PeaBrainC / CC BY-SA 4.0 (cropped)
- Mechanisms of Intracellular Calcium Responses: “Fluo-4 loaded HEK 293 cells” Borys Olifirov / CC BY 4.0 (cropped)
- Developing New Bioimaging Methods: “Microscope à fluorescence (Ifremer )” Olivier Dugornay / CC BY 4.0 (cropped)
- Related lab (Kano Lab): “Microscope stage set up for intravital microscopy” Aleanora / CC BY-SA 4.0 (cropped)
- Related lab (Minoshima Lab): “Femtosecond laser” Mina.ashrafzade / CC BY-SA 4.0 (cropped)
- Related lab (Maki Lab): “Firefly Luciferase Crystal Structure.rsh” Ramin Herati / Public domain (cropped)
- Related lab (Watanabe Lab): “Visa Dove Hologram Rainbow” Dominic Alves / CC BY 2.0 (cropped)
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